• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans

        互联网

        504
        RNA interference is a simple, efficient, and highly scalable method for the analysis of gene functions in Caenorhaditis elegans independently of mutants availability (1 ,2 ). It is used to study individual genes, gene families, and also for genomewide screenings (3 ). In a RNAi experiment C. elegans is exposed to double-stranded RNA that corresponds to the sequence of a specific mRNA. This triggers a series of enzymatic processes, resulting in a specific degradation of this target mRNA (4 –7). Consequently the animals display a phenotype that results from the depletion of the target protein. The RNAi phenotype can reach the severity of a null mutant (2 ). Although, generally RNAi is far more effective than antisense RNA (2 ,8 ) the effectiveness of a specific RNAi experiment depends on the target gene.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序