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        大肠杆菌常用菌株（Commonly used strains）

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        3500

        AG1

        endA1 recA1 gyrA96 thi-1 relA1 glnV44 hsdR17(rK - mK + )

        AB1157

        thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnV44(AS), galK2(Oc), LAM-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thi-1
         

        Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology (Edited by: F C Neidhardt J L Ingraham KB Low B Magasanik M Schaechter H E Umbarger). Washington, D.C., American Society for Microbiology 1987, 2:1190-1219.
        See CGSC#1157

        BL21

        E. coli B F- dcm ompT hsdS (rB - mB -) gal [malB+ ]K-12 (λS )

          • The "malB region" was transduced in from the K-12 strain W3110 to make the strain Mal+ λS . See Studier et al. (2009) J. Mol. Biol. 394(4), 653 for a discussion of the extent of the transfer.
        • Stratagene E. coli Genotype Strains

        BL21(AI)

        F– ompT gal dcm lon hsdSB (rB - mB - ) araB::T7RNAP-tetA

          • an E. coli B strain carrying the T7 RNA polymerase gene in the araB locus of the araBAD operonq .
        • Transformed plasmids containing T7 promoter driven expression are repressed until L-arabinose induction of T7 RNA polymerase.
        • Derived from BL21.
        • product page for more information.

        BL21(DE3)

        F– ompT gal dcm lon hsdSB (rB - mB - ) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

          • an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
        • Transformed plasmids containing T7 promoter driven expression are repressed until IPTG induction of T7 RNA polymerase from a lac promoter.
        • Wood, 1966 ) by transducing to Met+ .
        • See the original Studier paper or the summary in Methods in Enzymology for more details.

        BL21 (DE3) pLysS

        F- ompT gal dcm lon hsdSB (rB - mB - ) λ(DE3) pLysS(cmR )

        BNN93

        F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 mcrB e14-(mcrA- ) hsdR(rK - mK + ) λ- (责任编辑：大汉昆仑王)

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        纯化了20代的菌株还是没有测到同一种菌株。

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        问

        常用的蛋白纯化技术有什么？怎么在大肠杆菌、细胞中进行表达？

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