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        核酸标记技术检测法

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        四、 核酸标记技术检测法

          通过核酸标记技术可将细胞因子cDNA作为基因探钊检测细胞内细胞因子 基因组 DNA或mRNA。主要有以下几种方法:

        1.应用同位素(或非同位素)标记的cDNA探针,检测经Northern blot后细胞因子mRNA水平或采用打点杂交法。

        2.应用标记cDNA探钊与细胞或组织切片进行原位杂交,然后进行放射自显影。

        3.细胞因子mRNA经反转录为cDNA,用特异性细胞因子引物经聚合酶链反应(PCR)扩增细胞因子cDNA,Southern blot后用标记探针检测特异细胞因子DNA水平。

        (金伯泉)

        参 考 文 献

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