• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构：
    • 成为企业机构
    • 个人用户：
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Human Embryonic Stem (ES) Cell Protocols――General notes on ES cell culture

        互联网

        615

         


      • hES media has a two week shelf life.
      • hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended because it is very difficult to scrape cells in flasks.
      • hES cells need to be fed everyday with hES media. You can add 2 volumes of media and then not feed for 48 hours (ie. over the weekend.)
      • You should pick differentiated cells off of the plate if more than 5% of your culture is differentiated.
      • Alternatively, you can pick to keep the good colonies if there is massive differentiation within your culture.

        •  

        ad image
        ad image

        相关产品推荐

        Hemagglutinin/HA重组蛋白|Recombinant H1N1 (A/California/04/2009) HA-specific B cell probe (His Tag)

        Hemagglutinin/HA重组蛋白|Recombinant H1N1 (A/California/04/2009) HA-specific B cell probe (His Tag)

        ￥2570

        EmbryoMax™ 青霉素-链霉素溶液，100X，用于细胞培养, The EmbryoMax Penicillin-Streptomycin Solution, 100X is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications.，阿拉丁

        EmbryoMax™ 青霉素-链霉素溶液，100X，用于细胞培养, The EmbryoMax Penicillin-Streptomycin Solution, 100X is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications.，阿拉丁

        ￥174.90

        Albumin hydrolysate，for microbiological culture, from chicken egg white，阿拉丁

        Albumin hydrolysate，for microbiological culture, from chicken egg white，阿拉丁

        ￥5716.90

        Es Taq DNA Polymerase，EnzymoPure™, 500 U，阿拉丁

        Es Taq DNA Polymerase，EnzymoPure™, 500 U，阿拉丁

        ￥222.90

        IL-2重组蛋白|Recombinant Human IL2 Protein

        IL-2重组蛋白|Recombinant Human IL2 Protein

        ￥1080

        相关问答

        问

        我是用LPS诱导树突细胞成熟，结果细胞死亡，查了有不同的说明的，是不是一定要suitable for cell culture?

        4 回答 503 围观
        2023-08-08

        问

        Cell reports投稿难度如何？

        2 回答 1975 围观
        2022-10-14

        问

        小鼠效应T和记忆T cell

        1 回答 2217 围观
        2022-03-04

        相关方法

        大鼠ES的建系

        大鼠ES细胞的体外培养

        附着ES细胞分化培养

        推荐阅读

        Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells

        Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells

        Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells

        无忧采购 轻松科研

        无忧采购 轻松科研

        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序