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        Human Embryonic Stem (ES) Cell Protocols――General notes on ES cell culture

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      • hES media has a two week shelf life.
      • hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended because it is very difficult to scrape cells in flasks.
      • hES cells need to be fed everyday with hES media. You can add 2 volumes of media and then not feed for 48 hours (ie. over the weekend.)
      • You should pick differentiated cells off of the plate if more than 5% of your culture is differentiated.
      • Alternatively, you can pick to keep the good colonies if there is massive differentiation within your culture.

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