GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing
互联网
827
GABAA
receptor (GABAA
R) constitutes the main inhibitory receptor of the central nervous system. Due to the wide distribution and activity of its
main neurotransmitter agonist, the γ-aminobutyric acid (GABA), its pharmacology has been thoroughly studied, given rise to
the development of numerous drugs and of neuroactive compounds, some of the latest inducing neurotoxic effects.
In this chapter, we describe methods for studying the interaction of chemical agents with the GABAA
R and the effects they produce on its function, by using mice cortical and cerebellar granule neurons that have been grown
in vitro (cultured neurons). The methods described here include the evaluation of the binding of different agents such as
GABA agonists, GABA antagonists or allosteric modulators of the receptor, and the assessment of the receptor functionality
by analyzing the Cl−
flux induced by GABA.
