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TUBB3 (Neuronal Marker) Mouse

mAb(bsm-33177M)-50ul/100ul/200ul/500ul/200ug
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  • bsm-33177M
  • 2025年10月16日
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北京博奥森生物技术有限公司
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    规格：500ul产品价格：￥4000.0
    规格：200ug产品价格：￥5600.0
    产品编号bsm-33177M
    英文名称TUBB3 (Neuronal Marker) Mouse mAb
    中文名称微管蛋白β3（神经标志物）单克隆抗体
    英文别名Tubulin beta 3; beta III Tubulin; Tubb3; Tubulin beta-3; beta 4; MC1R; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Neuron-specific class III beta-tubulin; Syntaxin III; Neuron specific beta III Tubulin; Tubulin beta-4 chain; Tubulin beta-III; beta-4; CDCBM; CFEOM3A; M(beta)3; M(beta)6; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubulin beta 4; Tubulin beta-4 chain.
    中文别名TUJ1
    产品应用WB=1:1000-10000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, cerebellum, cerebellum, cerebellum, cerebellum, cerebellum, cerebellum, cerebellum, Human left parietal lobe, Human left parietal lobe, Human left parietal lobe, Human left parietal lobe
    抗体来源Mouse
    免疫原KLH conjugated synthetic peptide derived from human TUBB3
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    理论分子量50 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Microtubules

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > Tubulin

    Tags & Cell Markers > Cell Type Markers > Neuroscience Markers > Neuronal

    亚基Dimer of alpha and beta chains.
    亚细胞定位Cytoplasm, cytoskeleton.
    组织特异性Expression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues.
    翻译后修饰Some glutamate residues at the C-terminus arepolyglutamylated. This modification occurs exclusively on glutamateresidues and results in polyglutamate chains on the gamma-carboxylgroup. Also monoglycylated but not polyglycylated due to theabsence of functional TTLL10 in human. Monoglycylation is mainlylimited to tubulin incorporated into axonemes (cilia and flagella)whereas glutamylation is prevalent in neuronal cells, centrioles,axonemes, and the mitotic spindle. Both modifications can coexiston the same protein on adjacent residues, and lowering glycylationlevels increases polyglutamylation, and reciprocally. The precisefunction of such modifications is still unclear but they regulatethe assembly and dynamics of axonemal microtubules (Probable).
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, frommetaphase to telophase, but not in interphase. This phosphorylationinhibits tubulin incorporation into microtubules.
    相似性Belongs to the tubulin family.
    功能Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Neuronal MarkerBeta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

     

    应用推荐稀释比例
    {WB}{1:1000-10000}
    {IHC-P}{1:200-1000}
    {IHC-F}{1:200-1000}
    {IF}{1:200-1000}
    {Flow-Cyt}{1μg/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片7
    25 ug total protein per lane of various lysates (see on figure) probed with Tubb3 monoclonal antibody, unconjugated (bsm-33177M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片8
    25 ug total protein per lane of various lysates (see on figure) probed with TUBB3 monoclonal antibody, unconjugated (bsm-33177M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片11
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片12
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片13
    The U-87MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-TUBB3 antibody (bsm-33177M): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 7.1 }}] {Dai Nan. et al. DVL/GSK3/ISL1 pathway signaling: unraveling the mechanism of SIRT3 in neurogenesis and AD therapy. STEM CELL RES THER. 2024 Dec;15(1):1-18} {ICC} {Mouse}

    [IF={{ 5.923 }}] {Bing-Chun Liu. et al. Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells. Int J Mol Sci. 2021 Jan;22(14):7473} {ICC} {Human}

    [IF={{ 3.046 }}] {Yuyuan Ma. et al. Ultra-structural morphology analysis of human cranial bone marrow mesenchymal stromal cells during neural differentiation. Neurosci Lett. 2021 Aug;:136179} {ICC} {Human}

    [IF={{ 12.7 }}] {Fangyu Qiao. et al. Growth factor collected cell membrane-functionalized matrix for vascular-innervated bone regeneration. COMPOSITES PART B-ENGINEERING. 2025 Feb;291:112019} {IF} {Rat}

    [IF={{ 10.317 }}] {Yu D et al. MOF-encapsulated nanozyme enhanced siRNA combo: Control neural stem cell differentiation and ameliorate cognitive impairments in Alzheimer's disease model. Biomaterials . 2020 Oct;255:120160.} {IF,ICC} {Rat&Mouse}

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    TUBB3 (Neuronal Marker) Mouse mAb(bsm-33177M)-50ul/100ul/200ul/500ul/200ug
    ￥1180 - 5600