Evaluation of Nitrotyrosine-Containing Proteins in Blood Platelets

In this chapter we present two methods available to study the formation of nitrated proteins in blood platelets. We have used the methods in conjunction to first, identify proteins that become nitrated and second, to quantify the amounts of nitrated proteins present in platelets under a variety of different conditions. Since protein nitration is a new aspect of platelet biology we discuss some of the important findings in this field before outlining the methods.