• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Targeting Mutations to YACs by Homologous Recombination

        互联网

        517
        There are innumerable instances when one wants to manipulate cloned DNA: point mutagenesis for analysis of protein function and transcription factor binding sites, introduction of insertions to produce fusion proteins or to introduce reporter genes or motifs, and creation of deletions to define functional regions of proteins. One of the advantages of cloning in yeast artificial chromosomes (YACs) is that the DNA is maintained in the yeast host where one can carry out sophisticated and subtle mutagenesis with ease. Simple manipulations, such as the insertion of a mammalian selectable marker into the arm of the YAC, can be carried out by a single step integration or replacement as described in Chapter 17 . However, two rounds of recombination can be used to make more subtle mutations such as point mutations.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序