Genomic Reconstruction by Mitotic Recombination of YACs
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Mitotic crossing over was first observed in the somatic tissues of genetically marked strains of fruitfly (1 ), but has proved most valuable as a mapping tool in asexual fungi (2 ,3 ). Although the yeast Saccharomyces cerevisiae has a sexual cycle, and is therefore more amenable to genetic analysis by classical meiotic approaches, mitotic crossing over within a region of homology shared between two artificial chromosomes in this host has some interesting consequences, and can be used to physically reconstruct the genomic structure of an extended region in a single molecule (4 ).
