Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription
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The ability of oligonucleotides to bind double-stranded DNA in a sequence specific manner, forming a DNA triple helix structure, has led to a number of novel approaches in the study of various structural and functional properties of DNA in vitro and in vivo including transcription inhibition (1 –3 ), site-directed mutagenesis (4 ) and detection of DNA polymorphism (5 ).
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