Co-immunoprecipitation and Protein Blots

Knowledge about the identity of the interacting partners is important for the understanding of the function and the cellular activity of a given protein. Here we describe co-immunoprecipitation and pull-down as methods that are widely used for the identification and characterization of protein–protein interactions. These methods are well suited to find or confirm the interaction among multiple proteins, given the availability of a specific antibody for or a tagged version of the protein of interest.