Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes
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Purification of receptor proteins has always been an important and challenging task in advancing the structural characterization and also in providing sequence data for the molecular cloning of the recep- tors. Some of the difficulties in the purification of receptors are:
| 1. |
Low concentrations of receptors in the tissue;
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| 2. |
Solubilization of intact receptors from cell membranes; and
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| 3. |
Development of an efficient affinity chromatography system.
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