How to Assess the Intercellular Trafficking of Transcription Factors
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Non-cell-autonomous (NCA) control of plant development is an emerging field. Transcription factors (TFs) are the most important plant proteins involved in development and cell fate determination. In plants specialized intercellular symplastic channels, called plasmodesmata (PD), facilitate and regulate the NCA action of TFs. NCA-TFs move from cell to cell either selectively or non-selectively depending upon the specific interactions with PD or the pathway proteins. Here we describe different approaches to establish the role of TFs in NCA control of its function and the characteristic movement behavior.
