• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        PriCells: Isolation, culture, and purification of Human Pulmonary Arterial Smooth Muscle Cells

        互联网

        923

        PriCells: Isolation, culture, and purification of Human Pulmonary Arterial Smooth Muscle Cells (HPASMCs)

        1. HPASMCs were isolated from lungs resected from tissue of normal human or adult patients with malignant tumors.
        2. The arteries were separated from their adventitia and endothelium and then minced into 1- to 3-mm2 pieces with sterile scalpel blades.
        3. The tissues were mounted in tissue culture wells.
        4. The HPASMCs pellets were resuspended in Pricells Culture System and cultivated on 6-well plates pre-coated with gelatin (0.2%) for several days until subconfluent.
        5. Purification: The HPASMCs were isolated using magnetic beads (Dynal) according to the instructions of the manufacturer.
        6. The medium was replaced every 2 to 3 days, and the subcultures were obtained by trypsin/EDTA treatment of confluent monolayers at a splitting ratio of 1:3.
        7. Identification: Cells were identified with PriCells Identification System.

        PriCells: Primary cell products:

        1. Primary cell extract
        2. Primary cell RNA
        3. Primary cell DNA
        4. Primary cell base medium
        5. Primary cell growth supplement
        6. Primary cell isolation kit
        7. Primary cell identification kit
        8. Primary cell transfection kit

         

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序