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        Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications

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        With the completion of the sequencing phase of the Human Genome Project and the availability of fully sequenced clones containing intact functional loci from the various high-quality human P1- and F-plasmid derived/bacterial artificial chromosome (PAC/BAC) genomic libraries (1 ,2 ), it is becoming more convenient to study the expression and regulation of genes in their native genomic environment and to use such knowledge for therapeutic applications.
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