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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥16200.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥9720.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
AQPZUniprot No.:
P60844别名:
Bacterial nodulin-like intrinsic protein种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
27.7 kDa表达区域:
1-231aa氨基酸序列:
MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
CF Transmembrane Protein & Active Protein & In Stock ProteinBiological_Activity:
Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.Research Areas:
MicrobiologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验Strategies to Optimize Protein Expression in E. coli
., Su, X.Z., and Rawlings, C.A. 1995. Expression of recombinant feline tumor‐necrosis‐factor is toxic to Escherichia coli. Clin. Diagn. Lab. Immunol. 2:740‐746. Park, S.L
analysis of the Escherichia coli genome using the sequence‐to‐structure‐to‐function paradigm: Identification of proteins exhibiting the glutaredoxin/thioredoxin disulfide oxidoreductase activity. J. Mol. Biol. 282:703‐711
摘要 基因重组蛋白在大肠杆菌中表达时,由于表达量高,往往形成无生物活性的包涵体。包涵体必须经过变性和复性的过程才能获得有活性的重组蛋白。如何提高基因重组蛋白质的复性率,是生物工程技术的一个研究热点。对近年来的重组蛋白质的复性方法做一评述,为研究蛋白质折叠以及复性技术的进一步应用提供依据。 关键词 重组蛋白 包涵体 复性 二硫键 到目前为止,人们表达的重组蛋白质已有4000多种,其中用E.coli表达的蛋白质要占90%以上,尽管基因重组技术为大规模生产目标蛋白质提供
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