Expression of Foreign Genes in Acholeplasma laidlawii

互联网2014-02-10

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The expression of foreign genes in mollicutes has been difficult owing to inefficient transformation systems, and, until recently, by the lack of suitable cloning vectors. Few genes, excluding those associated with vector functions, have successfully been cloned and expressed in mollicutes (see Table 1 ; 1 –8 ).

Table 1 Genes That Have Been Cloned and Expressed in Mollicutes

Species	Vector	Expressed gene	Reference
Spiroplasma citri	SpV1	CAT gene from E. coli	*(1)*
Acholeplasma oculi	Tn4001	lacZ from E. coli	*(2)*
S. citri	SpV1	fragment of the M. pneumoniae P1 gene	*(3)*
Mycoplasma mycoides subsp. mycoides LC	p2D4, pIKΔ	Erythromycin resistance gene from streptococcus	*(4)*
A. laidlawii	pNZ19	Spiralin gene from S. citri	*(5)*
A. laidlawii	pGIP312	α-Amylase gene from Bacilius licheniformis	*(5)*
S. citri	pOT1	Spiralin gene from S. phoeniceum	*(6)*
Mycoplasma pneumpniae	Tn4001	Truncated HMW1 gene from M. pneumpniae	*(7)*
A. laidlawii	pNZ18	PS synthase gene from E. coli	*(8)*

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