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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Hydrogen peroxide-inducible genes activator (oxyR), Biotinylated
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥5370.0 |
| 规格: | 20μg | 产品价格: | ¥3324.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
oxyRUniprot No.:
P0ACQ4别名:
Morphology and auto-aggregation control protein种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
E.coli分子量:
82.1 kDa表达区域:
1-305aa氨基酸序列:
MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELGVMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTHFRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEAIRARMDGHFDKVLKQAV蛋白标签:
N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Hydrogen peroxide sensor. Activates the expression of a regulon of hydrogen peroxide-inducible genes such as katG, gor, ahpC, ahpF, oxyS (a regulatory RNA), dps, fur and grxA. OxyR expression is negatively autoregulated by binding to a 43 bp region upstream of its own coding sequence. OxyR is inactivated by reduction of its essential disulfide bond by the product of GrxA, itself positively regulated by OxyR. Has also a positive regulatory effect on the production of surface proteins that control the colony morphology and auto-aggregation ability.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验Production of Antimicrobial Substances by Lactic Acid Bacteria I: Determination of Hydrogen Peroxide
of ecological balance, mainly in pregnant women (10 ,11 ). The absence of these microorganisms is related to a higher risk of: bacterial vaginosis (12 ), recurrent urinary tract infections by Escherichia coli (13 ), and acquisition of human immunodeficiency
In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm
mutations that prevent export have been characterized (“down mutations”). Suppressors of these signal sequence mutations, known as prl mutations, have been isolated in most sec genes. More recently, inactive N-terminal regions of cytoplasmic proteins
Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli
We have developed four expression vectors to express antisense RNAs (asRNAs) by which genes of interest are silenced in Escherichia coli . The vectors are all IPTG-inducible and co-transformable in any combination and target genes
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