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        Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation

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        Stoichiometric and kinetic analyses are critical tools for developing efficient processes for the production of animal cell culture products such as therapeutic proteins or vaccines. This chapter describes the common analytical methods for quantifying animal cell growth and metabolism. It also presents sample calculations to illustrate the utility and limitations of various analytical methods. The application of calculated stoichiometric and kinetic parameters in mathematical models is also discussed. Hence, this chapter provides the basic tools for obtaining quantitative description of current cell culture state as well as applying the information for process optimization.
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