Preparation of Highly Sensitive Protein Array Using Reactive Polymer
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We have discovered a novel protein immobilization method, i.e., a “Three-Dimensional Nanostructured Protein Hydrogel” (3-D NPH), which is composed of protein-reactive polymer hybrid nanoparticles to detect protein–protein interactions. The 3-D NPH can be easily prepared by spotting a protein/reactive polymer mixture on a substrate.The resulting 3-D NPH is characterized by large amounts of immobilized proteins and a novel porous structure.
The 3-D NPH technology was applied to immobilize streptavidin (SA) onto Au-coated surface for surface plasmon resonance imaging (SPRi). By using 3-D NPH method, it was possible to improve the sensitivity of protein–protein interactions drastically comparing to the conventional protein immobilization method.
