Assay and Purification of Calmodulin-Dependent Protein Kinase
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Posttranslation covalent modification of proteins by phosphorylation represents an important mechanism in the control of many cellular functions (1 ,2 ). Protein kinases catalyze the transfer of the γ-phosphoryl group of ATP to an acceptor protein substrate. The activity of the enzyme is determined by the transfer of 32 P from [γ-32 P] ATP to protein substrate
