Procedure to remove spleen from mice
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OUTLINE
Experimental splenectomy in the mouse is used to study the role of the spleen in platelet destruction..
PROTOCOL
General anesthesia
Experimental splenectomy in the mouse is used to study the role of the spleen in platelet destruction..
PROTOCOL
General anesthesia
- inject i.p. 250µl/mouse of the ANESTHETIC mixture < wait for 2-10min for the anesthetic effect>
- for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet)
Preparation of the operation area
1. wash the surgical and adjacent area with C 2 H5 OH-70%
2. raze the fur with the razor
3. wash the surgical area with C2 H 5 OH-70%
Operation
- At the left hypochondrium make a 1.5-2cm "skin, muscles and peritoneum" incision with surgical scissors
- Gently trap and push the spleen free of adjacent tissue, separate its мlegо with blunt-ended scissors or forceps
- By a single knot tie off the artery with a 3-0 plane CATGUT by looping the suture through the mesentary (the artery enters the spleen at the superior polus). If necessary by a single knot tie off the efferent venule (exits the spleen at the inferior polus)
- Cut away the connective tissue and remove the spleen
- Apply on the open wound 1 pinch of Astrexene (Chlorhexidin HCl) (5mg/g)
- Apply on the peritoneum and muscles the мUо-like suture with plane CATGUT (3-0)
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Apply on the skin the мUо-like suture with ETHICON MERSILENE (3-0)
Wakening
1. inject 250ul/mouse of the "WAKENING MIXTURE"
Post operation period
1. after the operation took place let the animals to rest for at least 7 days in a cage with the filter
2. observe animals every day
SOLUTIONS
1. inject 250ul/mouse of the "WAKENING MIXTURE"
Post operation period
1. after the operation took place let the animals to rest for at least 7 days in a cage with the filter
2. observe animals every day
SOLUTIONS
- ANESTHETIC MIXTURE=mix 1ml of Domitor (Medetomidin HCl) (1mg/ml) , 1ml of Imalgene (Ketaminum HCl) (50mg/ml) and 8ml of PBS <all steril>.
-
"WAKENING MIXTURE"=mix 1ml of ANTISEDAN (Atipamezole HCl) (5mg/ml) and 9ml of PBS <all sterile!>
