Oligonucleotide Purification to Remove Trityl Group
互联网
	1. If the trityl group is not removed from a newly-synthesized oligonucleotide, it may interfere with polymerization reactions.
	
	2. Evaporate the oligonucleotide to dryness.
	
	3. Add 300 ml 80% Acetic acid. Incubate at 22 C for 20 min.
	
	4. Add 300 ml 100% Ethanol. Evaporate to dryness.
	
	5. If a white precipitate forms from volatile salts: resuspend in 300 ml ddH2O, add 300 ml 100% Ethanol. Evaporate to dryness.
	
	6. Resuspend oligonucleotide in 500 ml ddH2O. 











