Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography

Since DNA topoisomerases have become a major focus for scientists, the purification of these proteins from all sources represents one of the basic hurdles on the way to investigating this important class of enzymes. A variety of methods consisting of numerous steps have been applied to obtain a reasonable amount of pure enzyme (1 -4 ).