Systems for the Cell-Free Synthesis of Proteins
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We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli
. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale
setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given
in detail. We further provide specific protocols and parameters for the cell-free production of membrane proteins. High-throughput
screening applications of CF expression systems are exemplified as new tools for genomics and proteomics studies.
