• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        High-Throughput FACS Method for Directed Evolution of Substrate Specificity

        互联网

        790
        Directed evolution is a powerful technology for the isolation of enzyme variants with enhanced properties such as organic solvent tolerance (1 ), thermostability (2 ), glutaraldehyde resistance (3 ), and altered substrate specificity (4 ,5 ). Typically, enzyme variants are isolated using low throughput screening techniques such as agar plate or microtiter well plate assays. Recently, Fluorescence Activated Cell Sorting (FACS) has emerged as a tool offering significant promise for the high-throughput screening of very large libraries of mutants with single cell resolution (6 ). Arising from the simultaneous collection of multiple parameters, FACS is inherently capable of rapidly evaluating libraries for several different catalytic activities simultaneously with no increase in time required for library screening. We have exclusively used a Becton Dickinson FACSCaliber instrument, perhaps the most popular low-end sorter commercially available.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序