• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Immortalization Strategies for Mammalian Cells

        互联网

        600
        Mammalian cells normally have a limited life span and after having undergone approximately 30 population doublings stop dividing; this is known as senescence. However, when the control mechanisms which limit life span are modified cells can become immortalized i.e. have an unlimited growth potential (1 ). One such way of subverting these control mechanisms is to express an immortalizing oncogene in normal cells. These proteins override the normal control points and one of the most studied and best understood of this class of oncogenes is large T antigen which is contained within the genome of the monkey virus SV40 (2 ). SV40 large T antigen binds a number of proteins, but the most important are p53 and Rb which, when functioning normally, are negative regulators of cell division. Upon being bound by SV40 large T antigen, p53 and Rb are inactivated and the cells have an unlimited life span. There are a number of good reviews on immortalization (3 5 ) that deal in greater detail with biology involved in the process which is beyond the scope of this review.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序