• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Production of Conditioned Medium containing Wingless protein from S2 cells

        互联网

        753

         

        Production of Conditioned Medium containing Wingless protein from S2 cells

        - Grow heatshock wingless S2 cells at 25 degrees centigrade in a tissue culture flask to approximately 2.5 x 106 cells/ml.
        - Heat shock the cells at 37 degrees C for 30 minutes in the same flask
        - Move the cells back to 25 degrees for 40 minutes to allow the cells to recover
        - Remove the cells by pipeting, pellet them by spinning at 1200rpm and wash them 3 times with serum free medium to remove serum components.
        - Change medium to serum free (if desired, reduce the original volume by 10 fold to enrich for wg)
        - Collect conditioned medium 3-5 hours
        - Spin at 1200 rpm for 10 minutes to pellet the cells
        - Remove the medium and spin at 100,000 g's for 30 minutes at 4 degrees C to pellet any insoluble material
        - Remove the supernatant which contains soluble wingless protein. We concentrate the medium using Amicon Centricon 30 or Centriprep30 concentrators.
        - Either use immediately or flash freeze in liquid nitrogen and store and -70 degrees C

         

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序