Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning Microscopy

Confocal laser scanning microscopy (CLSM) is a useful research tool to explore the exopolysaccharides (EPS) in bacterial biofilms. Here, we describe the analysis of different biofilms of Myxococcus xanthus formed in a modified chamber slide system with CLSM. In conjunction with several specific fluorescent probes, the EPS within M. xanthus biofilms can be visualized and analyzed in situ.