Environmental Influences on Cells in Culture
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The technique of culturing cells derived from the nervous system has been in use for over 80 years, with the objective of simplifying the experimental system to provide readily manipulatable models of neural function. These preparations are useful for testing hypotheses relevant to cell adhesion, motility, survival, proliferation, longevity, and expression of cell type-specific properties. Many significant modifications of these methods have resulted over the years, as new data have emerged from cell biology and neurobiology studies (see reviews by Bottenstein, 1983a ,Bottenstein, 1985 ,Bottenstein, 1988 ).
