Calpain Activity in Rat Renal Proximal Tubules: An In Situ Assay
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Although considerable information about calpain has been obtained from studies using isolated enzyme preparations, this does
not permit the dynamic assessment of intracellular protease activity during changes in intracellular Ca2+
and pH. Intracellular calpain activity is regulated by complex interactions with the specific inhibitory protein (calpastatin),
Ca2+
and phospholipids, conditions which are difficult to duplicate in isolated systems. Thus a method to measure calpain activity
over time in living cells would be helpful in studying its regulation and physiological roles.
