实验动物的体重和周龄

ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and a ...

Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos. Procedure: We extractrf the gametes from the sea urchins fertilized the sea ur ...

Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external environment the experiment will determine if tempera ...

1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking at 4oC.2. Wash with 5 ml 0.1% BSA in PBS for 10 m ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in deficiencies in normal anterior-posterior developm ...

Introduction:The early stages of zebrafish Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only occurs in the blastodisc which is a thin region on t ...

Objective The objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation of physical defomities.IntroductionEmbryonic dev ...

BackgroundZebrafish Brachydanio rerio have a distinct embryonic development. The embryo goes through a process called epiboly. Epiboly as defined in the sixth edition of Developmental Biology(Gilbert ...

Background:Zebrafishor the teleost fish Danio reriois a rapidly developing organism that is a popular species for studying vertebrate development. Cleavage in the Zebrafish only occurs in the blastodi ...

I. Embryo Preparation 1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo by carefully grabbing and pulling with fine force ...

Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate gastrulation is the process by which the simple structure o ...

IntroductionIn early stages of development in amphibian embryos very few cells' fates are rigidly determined. However previous experiments show that cells in one area of the developing embryo are dete ...

From the various microsurgical procedures of Spemann and others several developmental principles have emerged about amphibian embryos. One of the most important discoveries was the understanding that ...

IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative development at some point during their growth. Regu ...

ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically we will split the morphogenetic field that is responsible for heart formation usin ...

BackgroundSince different amphibians execute gastrulation in different ways the study of amphibian gastrulation has been complex. A popular method used to examine and explore gastrulation and determin ...

Cyclopamine and jervine teratogens derived from the skunk cabbage Veratrum californicum are known to induce holoprosencephaly in chick embryos when introduced prior to gastrulation. Holoprosencephaly ...

The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that there are great similarities between avian and mammali ...

1. Use sterile technique. Prewarm Howard's Ringers in petri dish andagar/albumin culture dish to 37ºC.2. Crack 2-day egg into large sterile petri dish. Alternately open blunt endof egg and remove shel ...