结构基因组学

This CGH Protocol is used for DNA of good quality when available in sufficient amounts. We usually do replicate hybridizations using samples labeled "inversely" (reversing the label for test and norma ...

DAY 1:1) Reprecipitate DNA'sAdd the following DNA's to a 1.5 ml centrifuge tube mixing with pipet:20 ug Cot-1 DNA (~20ul)~200 ng Biotin labeled DNA (~10ul)~200 ng Digoxigenin labeled DNA (~10ul) Add 1 ...

Random Prime Amplification of DNA from small tumors (fresh or paraffin)We generally use 50 nanograms of fresh or paraffin microdissected Taqman-quantitated DNA (see microdissection protocol) for each ...

Cut 10-20X 10 um sections of formalin fixed paraffin samples into eppendorf tubes. Add 1 ml xylene mix incubate at 55 C for 15 mins. Release pressure spin down for 2 minutes in eppendorf ultramicrofug ...

In designing the PCR reaction be sure to include a negative PCR control (blank without DNA) a positive control (we use MPE-600 breast tumor cell line DNA) and enough reference DNA for all of the plann ...

Tissue Handling: Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear gloves lab coat etc). All plasticware (tubes tips etc.) should be RNase free and always handl ...

1. Prepare reaction mixtures per 50ul Add Enzymes last gently vortex mixture quick spin liquid to bottom of tube: LabelBiotin-dUTP Digox-dUTP FITC-dUTP Texas Red dUTP 10X Biotin dNTP5 ul 0 ul 0 ul 0 u ...

IntroductionThe technique of chromatin immunoprecipitation (ChIP) has proven to be a powerful tool allowing the detection of protein-DNA interactions in living cells. Hybridization of ChIP samples to ...

Comparative genomics has become a real tantalizing challenge in the postgenomic era. This fact has been mostly magnified by the plethora of new genomes becoming available in a daily bases. The overwhe ...

Gene Set Analysis and Network Analysis for Genome-Wide Association StudiesInti Pedroso and Gerome Breen INTRODUCTIONThe application of high-throughput genotyping in humans has yielded numerous insight ...

The software included are listed bellowIMPUTEa program for genotype imputation in genome-wide association studies and fine-mapping studies based on a dense set of marker data (such as HapMap) 5v1.0.0 ...

PLoS Genet. 2009 March; 5(3): e1000433. Published online 2009 March 20. doi: 10.1371/journal.pgen.1000433A Genome-Wide Association Study Confirms VKORC1 CYP2C9 and CYP4F2 as Principal Genetic Determ ...

Genome mapping and molecular breeding of tomato.--2007.pdf(1.5 MB 下载次数: 0 售价: 金钱 10 )摘要： The cultivated tomato Lycopersicon esculentum is the second most consumed vegetable worldwide and a we ...

从DNA水平上寻找确诊遗传病的指标或探讨遗传病和肿瘤的病因等方面，已取得很大成绩，这对产前诊断，早期确诊和突变基因携带者的检出等都有重要意义。所用的方法大体有以下几种。　　一、分离基因进行结构分析　　利用DNA离体转化，制备探针，制备基因文库进行筛检，最后鉴定载体中插入DNA片段的特性等一系列技术，可以设法分离出目的基因或某一特定的DNA顺序。然后通过DNA核苷酸顺序分析可以弄清楚某些疾病的病因。 ...

第七章 RFLP和RAPD技术第一节 概 述　　DNA分子水平上的多态性检测技术是进行基因组研究的基础。RFLP(Restriction Fragment Length Polymorphism限制片段长度多态性)已被广泛用于基因组遗传图谱构建、基因定位以及生物进化和分类的研究。RFLP是根据不同品种（个体）基因组的限制性内切酶的酶切位点碱基发生突变，或酶切位点之间发生了碱基的插入、缺失，导致酶切 ...

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Clonality - X Chromosome Inactivation AssayThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the fo ...

Clonality - X Chromosome Inactivation AssayThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the fo ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

PCR Protocol10X PCR Buffer1.0MgCl2 (2.0mM)0.8µldNTPS miundefined0.8µlPrimer-F (µg/µl)0.1µlPrimer-R (µg/µl)0.1µlTaq (5U)0.1µl32P dCTP (10µci/µl)0.1µlddH2O4.5µlDMSO (100%)0.5µlSUBTOTAL8.0µlTemplate DNA (50-10 ...