抗体应用

Since its discovery about 10 years ago, RNA interference (RNAi) has become an almost standard method for the knockdown of any target gene of interest. It is mediated by small interfering RNAs (siRNAs), which trigger a catalytic mechanism for mRNA degradation. Consequently, the delivery of int ...

RNA interference (RNAi)-mediated silencing of specific genes represents a powerful tool for analyzing protein function. It also has profound biotechnological applications for cellular engineering and therapeutics. However, it is necessary to have a method that controls RNAi in ...

Discovery of RNA interference (RNAi)-mediated specific gene silencing has raised hope for cancer therapy. Unfortunately, the execution of RNAi by delivering small-interfering RNA (siRNA) or small hairpin RNA (shRNA) remains a prime challenge. A methodical evaluation of cationic p ...

Linking genes with the underlying mechanisms of diseases is one of the biggest challenges of genomics-driven drug discovery research. Designing an inhibitor for any neurodegenerative disease that effectively halts the pathogenicity of the disease is yet to be achieved. The challen ...

We describe two efficient and inexpensive methods for reverse transfection with siRNA from a solid surface. One method involves localized reverse transfection from spots on a glass slide, which is mainly useful for making “transfection microarrays” (TMAs). The other involves reverse ...

Tumor invasion and metastasis are the primary causes of cancer patient mortality, underscoring the need for identification of novel genes and signaling pathways that mediate these prognosis-determining phenomena. To identify and characterize novel lung adenocarcinoma gen ...

siRNA therapy offers immense potential for clinical application. Under physiological conditions, however, siRNA was demonstrated to have a short half-life. Additionally, it may also cause ubiquitous gene silencing as it does not possess a tissue-specific homing mechanism. Thus, t ...

RNA interference, mediated by small interfering RNAs (siRNAs), is a powerful tool for investigation of gene functions and it is increasingly being used as a therapeutic agent. However, not all siRNAs are equally potent - although simple rules for the selection of good siRNAs were proposed early ...

The treatment of viral diseases such as HIV and HCV is limited by the genetic diversity of the viruses, especially when they are under the selective pressure of drugs. This problem holds true for gene-based therapies using RNAi in which there is evolution of drug-resistant strains under the discri ...

Currently, RNA interference technology is one of the most powerful tools in molecular biology and has been widely used in genetic manipulation. In addition to chemically synthesized small interfering RNA (siRNA), vector-based methods have been developed for stable gene silencing by t ...

RNA interference mediated by short interfering RNA (siRNA) molecules represents a powerful genetic tool with an increasing interest as potential therapeutics. Current bioinformatic approaches to design functional siRNA molecules take into account both empirical and ratio ...

RNA interference (RNAi) with small interfering RNA (siRNA) has become a powerful tool in functional and medical genomic research through directed post-transcriptional gene silencing. In order to apply RNAi technique to eukaryotic organisms, where frequent alternative splici ...

Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi ...

Cartilage explant cultures and chondrocyte cultures have been routinely used to study the degradative mechanisms involved in joint destruction that lead to disease states such as osteoarthritis and rheumatoid arthritis. Stimulation of these culture systems with catabolic cy ...

Inflammation, in its broadest sense, is a host response to tissue injury. The four ancient, cardinal, signs of inflammation are redness, heat, swelling, and pain. These clinical signs of inflammation are, of course, the macroscopic culmination of molecular and cellular processes, many of whi ...

Rheumatoid arthritis is characterized by inflamed joints with proliferating synovium and erosion of cartilage and bone. Eventually, loss of articular cartilage leads to a necessity for joint replacement surgery. Elucidation of the mechanisms of cartilage degradation are key to u ...

Exposure to pro-inflammatory cytokines (TNFα and IL-1) results in activation of signaling cascades that lead to stimulation of activity of the transcription factors AP-1 and NF-κB and induction of genes that are controlled by these transcription factors. Such genes code for cytokines (T ...

Nuclear transcription factors (TF) regulate tissue- and stimulus-specific gene expression through their ability to integrate extracellular signals at the level of the nucleus (1). Multiple human diseases, including cancer, cardiovascular disease, neurologic and autoimmu ...

Leukocyte adhesion to vascular endothelial cells is central in regulating inflammatory responses. Endothelial cells line the vasculature and cover over 1000 m2 in the average adult (1). These cells were long thought to be inert, acting only to contain the blood. This view began to change when Wa ...

The ability to measure and manipulate cytosolic free Ca2+ concentration is important for understanding many aspects of the physiology of inflammatory cells. The evidence for the role of this ion in triggering and controling neutrophils, monocytes, lymphocytes, and others cells rele ...