抗体应用

The delivery of drugs through the bloodstream in patients affected by central nervous system (CNS)-confined multifocal diseases can be therapeutically ineffective because of the presence of the blood-brain barrier (BBB), which forms an inaccessible wall to the majority of CNS-targ ...

This review deals with the principles and methods used to generate dendritic cells (DCs) from cord blood CD34+hematopoietic progenitors cells (HPC). Inasmuch as this culture system does not raise any particular technical difficulty, we will first thoroughly expose the principal cha ...

Hematopoietic stem cells (HSCs), progenitor cells capable of generating all peripheral blood cell lineages, are believed not to express lineage markers (Lin) found on the latter, nor CD38, but may or may not express CD34. Besides HSCs, more committed CD34+progenitors expressing CD38, and li ...

This chapter deals with the culture of hematopoietic stem cells contained in human umbilical cord blood in order to amplify the number of progenitor cells. The aim of expanding human umbilical cord blood cells (HUCBC) is to use a larger inoculum for hematopoietic stem cell (HSC) transplantati ...

A number of studies have demonstrated the feasibility of amplifying the short-term repopulating progenitors in vitro and have shown the relevance of ex vivo expansion approaches in hematopoietic transplantation (1-3) and gene therapy protocols (4-5). In spite of the beneficial effec ...

Cord blood (CB) transplantation is an increasingly used source of hematopoietic progenitor cells for allogeneic stem cell transplantation (1). At birth, blood sequestered into placental vessels can be recovered by draining the cord vein into a closed system. A mean recovery of 90 mL of place ...

This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human progenitor cells requires specific combinati ...

There has been great interest in ex vivo expansion of human long-term repopulating hematopoietic stem cells (LTR-HSC) for a variety of developing clinical applications including HSC transplantation, gene therapy, and production of mature blood cells in manufacturing. Because the d ...

Antigen-presenting dendritic cells (DCs) originate from hematopoietic stem cells in bone marrow through successive steps of differentiation (1-5). In peripheral tissues (e.g., skin), DCs are exposed to and capture antigens like viruses, bacteria, and other pathogens through endoc ...

Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) because of their ability to elicit strong proliferative response to alloantigens and to recall antigens. Most importantly, DCs have the unique ability to initiate the immune response by capturing antigens in pe ...

Dendritic cells are not a homogenous population, but develop through different differentiation pathways, whose prominence is dependent on the cytokine milieu. Although dendritic cells can be readily derived from monocytes, dendritic cells that develop from CD34+progenitor c ...

Dendritic cells (DCs) are the most potent inducers of immune responses and potent regulators of immunity. They act as sentinel cells in the peripheral tissues, playing a key role in the development of effective immune responses to different types of pathogens (1). At the same time, DC dysfunction m ...

Neutralizing antibodies are a critical component in the protection or recovery from viral infections. In the absence of available vaccines or antiviral drugs for many important human viral pathogens, the identification and characterization of new human monoclonal antibodies (h ...

Highly diverse antibody (Fab or scFv) libraries have become vital sources to select antibodies with high affinity and novel properties. Combinatorial strategies provide efficient ways of creating antibody libraries containing a large number of individual clones. These strate ...

Antibody libraries came into existence 15 years ago when the accumulating sequence data of immunoglobulin genes and the advent of the PCR technology made it possible to clone antibody gene repertoires. Phage display (most common) and additional display and screening technologies were ...

The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs, which have been utilized for decades, rabbit mAbs are ...

Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In support to the antibody-based therapeutics, the advent of recent technolo ...

The particular interest in VH antibody fragments stems from the fact that they can rival their “naturally occurring” single-domain antibody (sdAb) counterparts (camelid VHHs and shark VNARs) with regard to such desirable characteristics as stability, solubility, expression, and ...

We have developed a technology for the facile isolation of full-length IgG antibodies with desired specificity from combinatorial libraries expressed in Escherichia coli. Full-length heavy and light chains are expressed from a bicistronic operon and are secreted into the peripla ...

The development of high-throughput screening (HTS) technologies has become essential for initial characterization of recombinant antibodies and alternative affinity reagents, selected from large combinatorial libraries. Such binding ligands are routinely selected ...