其他模型

10X HC (7 Dropout Amino Acid Liquid)2.4 g tyr3.2 g ile2.0 g phe4.0 g glu8.0 g thr4.0 g asp6.0 g val16.0 g ser0.8 g argin final volume of 4L - Autoclave10X YNB58 g YNB (w/o amino acids and w/o ammoni ...

100X YC (7 Dropout Amino Acid Liquid)1.0 g arginine0.5 g aspartic acid0.5 g isoleucine0.5 g phenylalanine0.5 g proline0.5 g serine1.0 g threonine0.5 g valinein final volume of 100 mlstir with low hea ...

COMPLEX MEDIA YPAD medium or Also Called YPD plus AdenineYeast Extract - Peptone - Dextrose plus Adenine medium 6.0 gm yeast extract (Difco) 12.0 gm Peptone (Difco) 12.0 gm Glucose 60 mg adenine hem ...

Yeast Plates Hahn LabLast modified Tue Oct 6 1998Amino Acid Mix (40X) Use 0.6 g/literTyrosine 2 gArginine 4 gSerine 2 gValine 2 gThreonine 4 gIsoleucine 2 gPhenylalanine 2 gAspartic Acid 2 gProline 2 ...

GST Fusion Protein PrepItalics indicate optional steps especially useful for the analysis of untested proteins. GROWTH AND HARVESTING OF BACTERIAAdd 100 ul ampicillin to 100 ml LB. Inoculate and grow ...

GST Fusion Protein Purification from Yeast5 ml overnight culture of your favorite yeast in your favorite medium. Inoculate 50 ml and grow 30o C shaking O/N until OD600 = 0.8 to 1.2. For SCD cultures ...

YEAST TWO-HYBRID SCREEN WITH LIBRARY AND BAIT(The following protocol is for use with the LIBRARY transformation only)Day 1:Grow an overnight culture of a single colony of yeast transformed with bait ...

Table of contents1. Introduction and Background 2. Interaction mating 2.1 Interaction mating - small scale Protocol 1. Mating assay - small scale for tens of different bait or prey proteins. 2.2 Inter ...

Plasmid isolation from yeast (see Clontech YPH p31) Pick colonies into 0.5ml of SD-Leu (or other appropriate SD medium) Vortex for 1min Leave to grow O/N for 18-24h at 30°C 230-250rpm (best in 5ml bi ...

Rather Rapid Genomic PrepHoffman and Winston Gene 19871. Grow 5 ml yeast cultures to saturation.2. Collect cells by centrifugation and resuspend in 0.5 ml of water. Transfer cells to 1.5 ml microfuge ...

Yeast Chromosomal DNA Prepuse 25 ml cells per timepoint / sample add cells to 25 ml 100 % Ethanol (at -20 °C) add 1 ml 0.5 M EDTA store at -20 °C thaw cells wash once with H2O resuspend in 0.5 ml Shpe ...

Beta-gal AssayAdapted from Current Protocols in Molecular Biology1. Grow 5ml YEPD cultures to mid-log phase.2. Centrifuge and resuspend cells in 5ml Z-buffer then place on ice.3. Measure OD600.4. Use ...

Preparation of Yeast DNA Embedded in Agarose PlugsAnja van Brabant(adapted from Iadonato S. P. and A. Gnirke. 1996. RARE-cleavage analysis of YACs. Methods Mol Biol 54: 75-85.) 1. Inoculate a 5 ml cul ...

Beta-galactosidase Reporter Gene Assay (Liquid Form) REFERENCE: Hoffman G. Garrison T. R. and Dohlman H. G. Analysis of RGS proteins in Saccharomyces cerevisiae Methods Enzymol. 344:617-631 2002. ...

mTn-3xHA/GFP TRTn3 terminal inverted repeatsXaFactor Xa cleavage recognition siteloxRlox site target for Cre recombinaseGFPgene encoding Green Fluorescent Protein mutant p11URA3URA3 gene from S. cerev ...

PLATE Transformation"PLATE" is an acronym of the names of ingredients in the transformation solution: PEG Lithium Acetate Tris and EDTA.-Use sterile technique and sterile solutions throughout this met ...

Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18 19961. An average-size yeast colony (0.5-2mm) or a cell pellet from a liquid culture is touched with asterile ...

Typical transformation resultsThe following transformations were performed at the same time using the multiwell transformation protocol. Similar amounts of PCR product were used for each. The pictures ...

This Protocol allows for TRAFO with any Yeast cell sourceReferenece; Gietz R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.W ...

SMALL Scale Transformation 1. Inoculate 1 ml of YPDA or SD with several 2�3 mm colonies. 2. Vortex vigorously to disperse any clumps. 3. Transfer cells to a flask containing 50 ml of YPDA or SD: 4. I ...