其他模型

Embryo BuffersEgg Salts118 mM NaCl 48 mM KCl 2 mM MgCl2 2 mM CaCl2 10 mM HEPES (pH 7.4) ...

Cuticle preparationsThis procedure was adapted from one described over the phone (thanks P.G.). We're not sure if it appears elsewhere in published form.-Collect embryos on apple juice-agar plates for ...

GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES This is our lab protocol for growing imaginal disc cells (clone-8) The Milner Lab website has additional protocols for clone-8 cells. Cl ...

Medium Shields and Sang Medium 3 (SS3) modified for low serum. Supplemented before use with 2% heat inactivated FCS 2.5% FE2 12.5IU/100ml insulin and filter-sterilised through 0.22µm filter. Supplemen ...

SINGLE-FLY DNA PREPS FOR PCRGloor et al. 1993 Genetics 135:81-95We have developed a simple method for the rapid and reproducible isolation of DNA from single flies for amplification by the polymerase ...

IMMUNOHISTOCHEMISTRY ON Drosophila BRAINS Caution! In converting these protocols to HTML some units have been scrambled.Specifically microliters (ul) often appear as milliliters (ml). Think first! 1. ...

Labelling Drosophila pupae with BrdU ITO Kei 1992 OctoberCOLLECTING STAGED PUPAE 1. Prepare a couple of bottles containing late 3rd instar larvae which wander on the botlle wall (110-120 hours after e ...

Non-fluorescent protein/RNA double-labeling(a version of this is described in: Manoukian A. and Krause H.M.(1992) Genes Dev. 6: 1740-1751.)1. Collect embryos dechorionate and fix in a (1:1) mixture o ...

Single and Double FISH protocols for DrosophilaThis procedure is taken from a recently published book chapter. The reference is: Hughes S. and Krause H.M. (1998) Single and double FISH protocols for D ...

Yeast ProtocolsRather Rapid Genomic Prep Plasmid Rescue Protein Prep Yeast Histone Prep (from Jakob Waterborg; .pdf format) Media RecipesHC mediaHC Dropout Plate Supplementation Table YC mediaYC Dropo ...

June 1997 - optimized by N. Kertesz L.Leyns & E. De RobertisPlease cite: Belo J.A Bouwmeester T. Leyns L.Kertesz N. Gallo M. Follettie M. and De Robertis E.M. "Cerberus-like is a secreted factor with ...

Freezedown Guidelines What to SaveFor each 96-pack each lab will have yeast representatives from all four strains: BY4741 (or BY4730) BY4742 (or BY4739) BY4743 and the homozygous diploid. It is recogn ...

FEDEX Protocol(Or: How to increase the likelihood that your FEDEX packages reach their intended destination in a timely fashion)While this list seems pedantic so far it only incorporates actual bad ex ...

Sporulation and Dissection of Yeast a/alpha Diploids Linda HoskinsLast Modified Tue Nov 6 2001These conditions work well for the Hieter yeast strains1. Isolate diploid colonies. Inoculate 2 ml YPD + A ...

Alternate Sporulation Method for BY4743 Protocol: 1. Patch cells that have been freshly grown on YPD to freshly prepared GNA pre-sporulation plates for 1 day at 30°C.Repatch to another freshly prepar ...

Yeast Smash & Grab DNA Miniprep based on M.D. Rose F. Winston and P. Hieter (1990) Methods in Yeast Genetics: A Laboratory Course Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor New Yor ...

Phenol/Freeze RNA PrepSchmitt et al. (1990) NAR 18 3091-3092.DEPC is used to rid solutions of RNases. DEPC is very toxic and should be used only in the hood.Stuff you need:(DEPC treatment means: add D ...

Yeast Chromosomal DNA Prepuse 25 ml cells per timepoint / sample add cells to 25 ml 100 % Ethanol (at -20 °C) add 1 ml 0.5 M EDTA store at -20 °C thaw cells wash once with H2O resuspend in 0.5 ml Shpe ...

Materials required:Please Note: You Do Not have to Sonicate the Carrier DNA ANYMORE! WHY? I have done the experiments and shown that that Bigger is Better for Carrier DNA! Follow instruction below.Si ...

High efficiency LiAc transformationModified from Gietz Homepage.Stuff you need:10X LiAc 1 M LiAc filter sterilize50% PEG 3350 filter sterilize1. Inoculate 2-5 mls of liquid YPED or 10 ml synthetic med ...