Yeast Protocols

Yeast Protocols

• Rather Rapid Genomic Prep
• Plasmid Rescue
• Protein Prep
• Yeast Histone Prep (from Jakob Waterborg; .pdf format)
• Media Recipes
  
  • HC media
    
  • HC Dropout Plate Supplementation Table
  • YC media
    
  • YC Dropout Plate Supplementation Table
  • MET15 Resource center (lead plates)
    
• Sporulation and Examples of a Dissection
• Transformation
• Gene Disruption via PCR
• RNA Prep
• Quantitative Mating Assay
• Colony PCR
• Beta-gal Assay
• Yeast FACS Analysis
• Colony morphology
• Lab Lore
• Methods from the Fangman/Brewer Lab
• Methods from the Gietz Lab