其他模型

肺纤维化模型简介： 博莱霉素是具有多种抗肿瘤作用的多组分复合抗生素，其毒副作用之一是引起肺纤维化。由于病理组织学改变与人类肺纤维化最为接近，故被广泛用于诱导肺纤维化模型。 气管内给药是最为常用的给药途径，方法是是BLM进入动物气管后立即通过直立旋转等手段，使药物均匀分布于肺组织，导致肺部病变发生PF。气管给药可以是一次性的，也可以是重复多次的。其中使药物进入动物气管内有三种方法：1.直接支气管插管，然后滴入BLM。2.麻 ...

急性肾损伤（acute kidney injury，AKI）是临床上较为常见的急危重病症之一，常见于大手术后、创（烧）伤、危重病等患者中，可导致患者电解质紊乱、酸中毒及容量负荷过重，并最终可发展成为尿毒症。肾缺血再灌注（renal ischemiareperfusion，RIR）损伤是由各种原因导致的肾脏灌注不足所致，伴随着一系列连贯的细胞事件发生，包括活性氧 (ROS) 释放、凋亡、坏死、炎症细胞的浸润和活性介质的释放，从而导致组织损伤 ...

开发实验设计助手的Nathalie Percie Du Sert图片来源：Welcome Trust应该有一种免费在线工具，可以让动物实验设计可视化，并提出重要的反馈，这样可以把科学家从糟糕的研究设计歧路上拉回来。这正是一些软件研发者所希望的目标。过去几年，研究人员在发表的动物研究成果中挑选出大量的设计和报告错误，他们指出这些可能会导致偏见。作为回应，数百家期刊就报道动物研究论文达成自愿性指导规则，形成最佳操作实践清单，如利 ...

1、实验器材（从左到右）：第一行：干棉球、酒精棉球、10％水合氯醛＋注射器、碘伏＋棉签第二行：三种不同粗细的鱼线（鱼线最好在2.0、2.5mm处各用记号笔作好标记，便于观察进入距离）、弯盘内为手术器材第三行：记号笔、自制拉钩（皮筋+曲别针＋大头针）2、麻醉：可用饮料瓶自制捕鼠器（适用于不敢手抓大鼠的），用于打麻药，根据大鼠的重量选择不同粗细的饮料瓶（太粗了大鼠可在瓶中回头）。效果图3、麻倒后绑在手术台上4、剪去颈前的鼠毛，碘伏消毒。5 ...

器材：充裕版：通风橱、手术剪、小止血钳、眼科镊、眼科剪、小咬骨钳、手术刀柄及刀片简易版：手术剪、小止血钳、眼科镊、眼科剪（这次操作用的这套东西）1. 剥离颅骨及脊柱周围的肌肉2. 由脊髓下段的缺口开始咬开并去除椎板3. 暴露出整个脊髓背侧4. 眼科剪剪开脊髓周围的脊膜及背根节，剥离出脊髓5. 同上6. 剪去残余的椎骨7. 由枕骨大孔处入路掀开咬除颅骨8. 继续9. 继续10. 暴露嗅球11. 咬除侧面的骨板，清理小脑绒球周围的组织，剥离出绒球12. 清理小脑绒球周围的组织 ...

miRNA动物实验方法简介：miRNA作为生命科学最为热门的研究领域之一，动物实验已经成为阐明miRNA自身功能不可或缺的关键环节，也是搭建基础研究到临床应用的桥梁，其重要性正日益显现。动物实验影响因素多，难度大，成本高。为了解决广大科研工作者面临的这些难题，锐博生物利用自身多年积累的先进技术，成功开发出国际一流品质miRNA研究工具：micrONTM miRNA agomir和micrOFFTM miRNA antago ...

image of a whole mount from a 4 wk old virgin ProtocolSpread tissue on glass slideFix in Carnoy's fixative for 2 to 4 hours at r.t.Wash in 70 % EtOH for 15 minChange gradually to distilled waterRinse ...

Sepharose 4B-CL Chromatography BAC DNA Purification for Transgenic ProductionThis method was contributed by Wes Dunnick at the University of Michigan Medical School in Ann Arbor Michigan. The approac ...

RNA Whole Mount In Situ HybridizationCore protocol for both mouse and chick embryos Cepko/Tabin lab Embryo preparationFix embryos in fresh 4% paraformaldehyde (or a thawed frozen aliquot) in PBS at 4 ...

Preparation of Copy Standards for PCR GenotypingPCR screens must be designed to detect transgene DNA at the single copy level. To demonstrate this level of sensitivity non-transgenic tail DNA is spike ...

AP Buffer: 100 mM Tris-HCl pH 9.5 100 mM NaCl 10 mM MgCl2 PTM: PBS 0.1% Tween-20 2 mM MgCl2 Since this is generally done in conjunction with lacZ staining embryo/tissue is usually fixed as per lacZ st ...

DETECTION OF ß-GALACTOSIDASEThe E. coli lacZ gene encoding ß-galactosidase (ß-gal) is the classical histochemical reporter gene (Beckwith 1980). It can be detected using a variety of substrates all o ...

Dauer Pheromone PrepAuthors:From Cori Bargmann and Brenda Reinhart 1992A variation of Riddle's protocol: Small molecule prep-1. grow up worms super dense1 liter of culture of worms in s-basal and e.co ...

Injection of C. elegansPrepare injection padsline up three 22x50 mm coverslips place drop of molten 2% agarose on each quickly place slide on top of the agarose and wait 20 seconds (see figure) carefu ...

Worm Injectionspads:melt 2% agarose in water keep molten at 42oCuse 25mm x 25mm or larger microscope slidesspread out microscope slides onto a heat resistant glass or metal trayusing a pasteur pipet p ...

oocyte isolation Anethesize female using benzocaine - put ~5ml 6% benzocaine (stock solution in 100% ethanol) into 1L water.- leave animal until it is limp and completely unresponsive.- place animal o ...

Chi's method Pick your embryos (~5 per tube) and place in an autoclaved 1.5 ml capped centrifuge tube Wash embryos 1 ml of autoclaved DEPCed-PBS (containing 1x PBS; pH 7.4) -- take care not to lyze or ...

Oocyte transfer: IntroductionProtocol submitted by: This chapter describes a method that has been used successfully to study the roles of a number of maternal mRNAs in Xenopus embryos (Kofron et al. 1 ...

1-2 g fresh material freezer-dried ground with 0.2g sand (if necessary) and then homogenized with 10ml RNA extraction buffer (see below). Spin at 8000rpm 4oC for 10 minutes Remove the supernatants to ...

Overview Using this procedure it is possible to follow the development of pollen mother cells through to mature pollen. Sterile males were made using irradiation mutagenesis of Landsberg erecta seed ...