• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购

        化学生物学实验技术

        丁香实验推荐阅读
        The Flexible Pocketome Engine for Structural Chemogenomics

        Biological metabolites, substrates, cofactors, chemical probes, and drugs bind to flexible pockets in multiple biological macromolecules to exert their biological effect. The rapid growth of the structural databases and sequence data, including SNPs and disease-related gen ...

        丁香实验推荐阅读
        Network and Pathway Analysis of CompoundProtein Interactions

        We describe an integrated system that brings together predictive chemical analyses based on compound structure, knowledge bases of chemogenomics data associating compounds to biological, pharmacological and toxicological properties, and a systems biology functional d ...

        丁香实验推荐阅读
        SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems

        In eukaryotic cells, the reversible attachment of small ubiquitin-like modifi er (SUMO) protein is a post-translational modifi cation that has been demonstrated to play an important role in various cellular processes. Moreover, it has been found that SUMO as an N-terminal fusion partner e ...

        丁香实验推荐阅读
        Inhibition of the SUMO Pathway by Gam1

        We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manip ...

        丁香实验推荐阅读
        Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay

        In this chapter we describe a novel, sensitive, homogenous high throughput reporter-based in vitro assay for SUMO protease activity developed by Progenra, Inc. A reporter construct was created by fusing His6-tagged small ubiquitin-like modifi er (SUMO) to the amino terminus of the report ...

        丁香实验推荐阅读
        FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities

        In humans cells three SUMO paralogues (SUMO-1, SUMO-2 and SUMO-3) and six SUMO specific proteases (SENP1-SENP3 and SENP5-SENP7) are expressed. Together the SUMO proteases perform three distinct functions. They: (1) process the immature pro-SUMO proteins into the active forms, (2) remove S ...

        丁香实验推荐阅读
        An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage

        To measure rates of sumoylation and isopeptidase cleavage in vitro, we developed an enzyme assay that is based on fl uorescence resonance energy transfer (FRET). FRET is a process by which the excited state energy of a fl uorescent donor molecule is transferred to an acceptor molecule. Effi cient en ...

        丁香实验推荐阅读
        Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates

        SUMO proteases catalyze two reactions, deconjugation of SUMO from substrates and processing of precursor SUMO isoforms to prepare SUMO for conjugation. The SUMO protease family includes two members in yeast (Ulp1 and Ulp2) and as many as six members in human (SENP1-3, SENP5-7). SENP/Ulp prot ...

        丁香实验推荐阅读
        Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

        Fluorous-based carbohydrate microarrays provide an alternative to traditional covalent microarray �platforms for probing protein–carbohydrate-binding interactions. The most studied plant lectin, concanavalin A (ConA), is known to bind to terminally α-linked mannos ...

        丁香实验推荐阅读
        General Procedure for the Synthesis of Neoglycoproteins and Immobilization on Epoxide-Modified Glass Slides

        Neoglycoproteins, such as BSA-glycosides, contain carbohydrates covalently attached to a protein carrier via nonnaturally occurring linkages. These conjugates have been used for decades to study carbohydrate–protein interactions and are frequently used as immunogens to ...

        丁香实验推荐阅读
        Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays

        Our experience in coating of solid surfaces with glycans, mainly for obtaining routine glycoarrays based on immunological plates, is summarized. Three polystyrene coating techniques are described: direct physical adsorption, covalent binding, and immobilization using the b ...

        丁香实验推荐阅读
        Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray

        Monitoring multiple biological interactions in a multiplexed array format has numerous advantages. However, converting well-developed surface chemistry for spectroscopic measurements to array-based, high-throughput screening is not a trivial process and often proves ...

        丁香实验推荐阅读
        Glycoarray by DNA-Directed Immobilization

        Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the i ...

        丁香实验推荐阅读
        Fabrication of Carbohydrate Surfaces by Using Non-derivatised Oligosaccharides

        Surface-based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate–protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry ( ...

        丁香实验推荐阅读
        Polysaccharide Microarrays: Application to the Identification of Heparan Sulphate Mimetics

        The interaction of polysaccharides with proteins modulates or triggers many biological effects. In particular, heparan sulphate proteoglycans (HSPGs) have multiple regulatory interactions with growth factors, enzymes, enzyme inhibitors, and some components of the extra ...

        丁香实验推荐阅读
        Carbohydrate Antigen Microarrays

        This chapter describes one of my laboratory’s working protocols for carbohydrate-based microarrays. Using a standard microarray spotter, we print carbohydrate antigens directly on the nitrocellulose-coated bioarray substrates. Because these substrates support nonco ...

        丁香实验推荐阅读
        MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays

        Glycan-processing enzymes such as glycosyltransferases and glycosidases are responsible for the makeup of the glycome. The definition of their substrate specificities is, therefore, a central task in glycomics. In addition, these enzymes are themselves useful synthetic tools f ...

        丁香实验推荐阅读
        Probing VirusGlycan Interactions Using Glycan Microarrays

        Glycan microarrays are surfaces that contain immobilized oligosaccharides or glycoconjugates and have proven useful in probing the interactions between glycan-binding proteins (GBPs) and individual glycans. Such glycan microarrays have been especially important in st ...

        丁香实验推荐阅读
        Recent Advances and Future Challenges in Glycan Microarray Technology

        Glycan microarrays, carrying hundreds of different sugars on chip surfaces, have become a standard tool for the study of interactions of biomolecules with carbohydrates. The chip-based format offers important advantages, including the ability to screen in parallel several thous ...

        丁香实验推荐阅读
        Microarray Technology Using Glycans Extracted from Natural Sources for Serum Antibody Fluorescent Detection

        Glycan microarray technology enables the screening of large numbers of glycan samples for glycan–protein interactions, based on the presentation of immobilized glycans in a discrete pattern on a solid support. Here we describe a glycan microarray approach employing glycans enzym ...

        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序