细胞功能测定

Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety complete with figures. To reference this material please in ...

Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela J. Fraker Michian State University East Lansing M ...

Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela J. Fraker Michian State University East Lansing M ...

Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a goo ...

Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a goo ...

FACS Analysis Using Peripheral Blood CellsCollect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix immediately to prevent clotting. Keep tubes on ic ...

Materials Monolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used or cultures of tetrahymena yeast or algae may be used.)H-thymidine with at least 4 µc/ml 0.36 c/mMPhosp ...

Thawing Cells (Schreiber's protocol)Thaw vial quickly in 37°C water. Caution - vial can explode. Transfer cells to sterile 15 mL centrifuge tube. Add 50 µl warm FBS (fetal bovine serum heat inactivate ...

Working Cell BankAuthor: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Provides detailed protocol for establishing a working cell bank PurposeTo describe the preparation of a Working Cell ...

Tissue Culture - Thawing Cells from Liquid NitrogenMETHOD:Prepare before thawing:Fill a test tube of 10 - 15 mls of cold media (appropriate for your cell line) with 10 - 20% fetal calf serum (use 2x t ...

Tissue Culture - Thawing Cells from Liquid NitrogenMETHOD:Prepare before thawing:Fill a test tube of 10 - 15 mls of cold media (appropriate for your cell line) with 10 - 20% fetal calf serum (use 2x t ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction21.1.1Terminology of cell death 21.1.2Differences between necrosis and apoptosis31.1.3Apoptotic Pathways4 1.2 Apoptosis Assay Methods6 Me ...

Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction21.1.1Terminology of cell death 21.1.2Differences between necrosis and apoptosis31.1.3Apoptotic Pathways4 1.2 Apoptosis Assay Methods6 Me ...

Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during a ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. WilcoxJosé C. Rodriguez and Héctor de Léon - Emory University - April 1996P ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. WilcoxJosé C. Rodriguez and Héctor de Léon - Emory University - April 1996P ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. Wilcox andJosé C. Rodriguez - Emory University - April 1996Protocol for Fro ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. Wilcox andJosé C. Rodriguez - Emory University - April 1996Protocol for Fro ...