细胞功能测定

Isolation of Lung Bud Endoderm What you need: E11-12 mouse embryos DMEM with 5% fetal bovine serum petri dishes for dissections and washes Tyrode-Ringer's solution pH 7.6-7.7 (recipe below) pancreatin ...

FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose no pyruvate) 7% FBS and 1X ...

In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. Over the last ...

Cloning by Limiting Dilution of HybridomaAuthor: Nanci DonackiSource: Contributed by Nanci DonackiMaterials DMEM high glucose (Life Technologies Inc. #10313-021 or equivalent)Fetal Bovine Serum (Li ...

CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Feta ...

CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Feta ...

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...

Mixing F-12 (HAM) Media1) Start by rinsing a graduated cylinder three times ddH2O. 2) Then fill the cylinder to 600-ml with ddH2O. 3) Add one bottle of F-12 (HAMS) (Sigma #N-4388). 4) Stir this until ...

Culturing Chick Embryonic Cardiac Myocytes and FibroblastsMaterials1. Culture medium: F12K without glutamine 3% FCS 100 units/ml 100 ug/ml streptomycin (myocyte medium also for mixed culture); and F12 ...

Culturing Chick Embryonic Myoblasts/Myotubes and FibroblastsDay 1Materials1. 100 ug/ml gelatin (described separately later). Procedure1. Pipet 10 ml gelatin onto each of two 100 mm culture dishes. Lea ...

Human Microvascular Endothelial CellsCC-2505HMVEC-d NeoDermal - Neonatal500000 cells/ampCC-2516HMVEC-d NeoDermal - Neonatal(Pooled from500000 cells/ampseveral donors)CC-2543HMVEC-d AdDermal - Adu ...

Human Microvascular Endothelial CellsCC-2505HMVEC-d NeoDermal - Neonatal500000 cells/ampCC-2516HMVEC-d NeoDermal - Neonatal(Pooled from500000 cells/ampseveral donors)CC-2543HMVEC-d AdDermal - Adu ...

Lacrimal Experimental Protocol (4/4/94)Day Before: 1. Prepare (6) 50 ml conicals each containing 50 ml of DMEM (high glucose) and gentamycin (50 µg/ml); place in incubator (90% O2 10% CO2).2. Thaw FBS ...

MN Analysis : Blood Lymphocytes MN in Human Lymphocytes (method description)Lymphocyte isolationLymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted 1:1 with phosphat ...

MN Analysis : Blood Lymphocytes MN in Human Lymphocytes (method description)Lymphocyte isolationLymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted 1:1 with phosphat ...

ES Cell Culture and ManipulationCulturing ES cellsMedia High glucose DMEM (-pyruvate -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type be sure!!)1X l-glutamine1X Penicillin/stre ...

ES Cell Culture and ManipulationCulturing ES cellsMedia High glucose DMEM (-pyruvate -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type be sure!!)1X l-glutamine1X Penicillin/stre ...

b-Galactosidase Color Assay for Cultured Cells 1. For transient expression assays wait until 2 days after the transfection to begin the color assay. Aspirate the media and rinse the cells twice with ...

b-Galactosidase Color Assay for Cultured Cells 1. For transient expression assays wait until 2 days after the transfection to begin the color assay. Aspirate the media and rinse the cells twice with ...

Basic Method for Indirect Immunofluorescence Labeling BackgroundThis is the method for indirect immunofluorescence labeling; that is the antibodies do not have the fluorescent dye attached. Indirect l ...