细胞功能测定

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...

Growing cells No two cell lines behave exactly the same so you must learn the peculiarities or personality of each of the cell lines with which you work. Irrespective of cell type all cells are dilute ...

This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program developed by Becton Dickinson. The problem that follow are presented in increasing degress of co ...

This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program developed by Becton Dickinson. The problem that follow are presented in increasing degress of co ...

Infection with retroviruses It is well established with avian retroviruses that cells are most efficiently infected just after they are trypsinized. Trypsinization does two things. First it apparently ...

Establishment of a Primary CultureLEVEL III Materials Chick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors forceps and probesSterile petri platesPhosphate buffered sal ...

Establishment of a Primary CultureLEVEL III Materials Chick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors forceps and probesSterile petri platesPhosphate buffered sal ...

Staining protocols for placental explant culturesWhole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining but it can be extended to double antibody staining. ...

From early post-implantation stages to approximately 18 weeks' gestation cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate to form columns. From the distal edges of ...

From early post-implantation stages to approximately 18 weeks' gestation cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate to form columns. From the distal edges of ...

1 Basic Techniques - The ?Do?s and Don'ts? of Cell CultureGiven below are a few of the essential "do?s and don?ts" of cell culture. Some of these are mandatory e.g. use of personal protective equip ...

Components and Supplementation of Various Culture MediaAbbreviations: DMEM = Dulbecco's Modified Eagle's Medium FCS = Fetal Calf (or Bovine) Serum GPS = Glutamine-Penicillin-Streptomycin BME = b-Merca ...

Recipes for Tissue Culture Selective Drug Stocks100X G418 (Geneticin): Obtained as crystalline powder from GIBCO (cat.# 860-1811) and stored @ room temp. in T932. The amount of active ingredient per ...

Serum Thawing & Heat Inactivation(Chris Cohick from JRH Biosciences catalogue)How to thaw serum:Serum that is stored at -10º C to -40º C is stable for extended periods of time. It is neither necessary ...

Solutions for Tissue/Cell DigestionTrypsin (13 ug/ml) in Saline GMaterialsWorthington 3x crystallized trypsin (LS03707) 1.3 mg. Sterile Saline G (pH 7.0) 100 ml. Procedure (all steps on ice)1. Dissolv ...

Stock solutions for tissue culture The kitchen makes Tris TD Tryp/TD PBS and VE.Tris is a fairly complex Tris-buffered physiological saline solution. It is used to wash cells and is preferable to TD u ...

Tissue Culture Media We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a res ...

Tissue Culture Media We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a res ...

ORGANOTYPIC KIDNEY CULTURES -embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. -metanephroi and associated ureteric buds are microdissected and placed in holding medium (L ...

ORGANOTYPIC LIMB CULTURES -embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c. -limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ ser ...