常用设备/其他

大家在实验过程中越来越多的需要用到光学系统，从一般的照相，到凝胶成相，分光光度仪，酶标仪，化学发光仪，荧光PCR，测序系统，流式细胞仪等等等等，都会涉及到光学系统，那么你到底了解多少光学系统呢。我们先从最基本的开始，准备好，开始复习高中课程了。光的原理�光波（Light Wave）��光由相互垂直的电场和磁场的振动并向空间传播的一种电池波。其传播速度在真空中，为每秒三十万公里。光波相邻的波峰或波谷 ...

Leica珍藏照相机1925年前，位于德国Wetzlar的精密光学仪器制造商Ernst Leitz公司将35mm电影胶片用在照相机上，开创了摄影领域的新纪元，这就是Leica。这是一种完全不同于以前折叠式木头制造的照相机，整个机架都是用金属制造，是世界上第一台精密的35mm照相机。由于轻巧和方便使用，而且配备有优质的镜头，所以很快受到了全世界的摄影发烧友和用户的喜爱。Leitz的员工对公司的生产 ...

一、核酸及蛋白质常用数据 1．核苷三磷酸的物理常数化合物分子量λmax(pH7.0)1摩尔溶液（pH7.0）中λmax时的最大吸收值OD280/OD260ATP507259154000.15CTP48327190000.97GTP523253137000.66UTP484262100000.38dATP494259152000.15dCTP46727193000.98dGTP50725313700 ...

化学元素及原子量（69年国际原子量）名称　　　符号　　　　原子量　　　　名称　　　　符号　　　原子量氢　　　　　　H　　　　　 1.008　　　　　碳　　　　　 C　　　　　 12.011　氮　　　　　　N　　　　　14.0067　氧　　　　　　O　　　　　15.9994 钠　　　　　　Na　　　　 22.9898　镁　　　　　　Mg　　　　　24.305 磷　　　　　　P　　　　　 30.973 ...

Exposing gels and plates containing radioactive samples to X-ray film Although most people use the PhosphorImager for western blots kinase assays and methionine-labeled samples X-ray film remains the ...

Autoradiography and Intensifying ScreensFrom time to time someone asks a question about intensfying screens both in my lab and in these web forums (like why do you put them in the deep freeze?). Recen ...

CentrifugationThe single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics optics and mathematics by T. Svedberg and J ...

Centrifugation TheoryCentrifugation is a process used to separate or concentrate materials suspended in a liquid medium. The theoretical basis of this technique is the effect of gravity on particles ( ...

Rotor equilibration without counterbalanceContributed by Dr.A.GratchevThis is relatively obvious method which I found not used by most of the researchers. Usually the equilibration of 234689 and 10 tu ...

Radioactive DecayIsotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable however radioisotopes are unstable and disintegrate ...

A. Gilson Pipetmen Autopipets (and similar models)We primarily use the Gilson autopipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The va ...

I. Safety Procedures A. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by law to supply the user with pertinent information ...

Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors the main disadvantage is ...

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide. Decontamination of solutions 0.5mg/mlThis method reduc ...

Log in radioactive material received and deduct amount of radioactive material used in each experiment in Radioisotope Log Book. Clear your bench top work area and make sure it is clean. Put on your l ...

RULES FOR THE USE OF RADIOACTIVITY ...

Silane and Poly-L-Lysine Coating of Microscope Slides1) Wash slides in Decon solution for 30 minutes. 2) Wash slides in running tap water for 30 minutes. 3) Wash slides in distilled water for 2 x 5 mi ...

This program helps you to calculate the concentration of nucleic acids according to their optical density. (DNA RNA oligonucleotides). It is better to use {OD260-OD320} instead of OD260. For a series ...

Plate assay for determination of DNA concentrationA fairly accurate rapid assay of DNA concentration can be obtained by UV visualization of samples spotted onto ethidium bromide-containing agarose pla ...

Characterization of dnaLEVEL I Materials DNA sampleSSC bufferUV spectrophotometer 3 and quartz cuvettes Procedure Dissolve a small quantity of your extracted DNA in 3.0 ml of 0.1X SSC. Turn on and bla ...