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HISTOLOGICAL FIXATIVES

Fixation: Confers chemical stability on the tissue Hardens the tissue (helps further handling) Halts enzyme autolysis Halts bacterial putrefaction May enhance later staining techniques Introduces a 'c ...

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Processing of Microdissected Tissue for Molecular Analysis

These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ...

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Slide Preparation for Laser Capture Microdissection (LCM)

These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ...

丁香实验推荐阅读
Processing of Microdissected Tissue for Molecular Analysis

These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ...

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Slide Preparation for Manual Microdissection

These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research ...

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Microwave Tissue Processing

PURPOSE: To provide rapid tissue processing that will decrease turn-around-time.PRINCIPLE: Microwave exposure is used to dehydrate clear and impregnate tissue samples. Fixation of tissue samples is ac ...

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Microwave Processing Schedules for Biopsies

Procedure IIIUsed by Denise Hardy ARUP Laboratories Salt Lake City UT. Pre-fix room temperature 1 hour. Microwave in reagent alcohol 67°C 5 minutes. Microwave in 100% isopropyl alcohol 74°C 5 minutes ...

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10% FORMAL SALINE

Wear appropriate protective clothing eg. laboratory coat gloves and goggles.Weigh out 900g of sodium chloride and dissolve in warm water.Unscrew cap on porthole of formalin mixing tank (beneath cut-up ...

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Microwave Processing Techniques for Microscopy

How does a microwave oven work?Microwaves are electromagnetic waves. Electromagnetic waves can be classified by their frequencies and include radio waves television signals radar beams infrared waves ...

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4% PARAFORMALDEHYDE

4% PARAFORMALDEHYDE/PBS 1. Measure 100ml Phosphate Buffered Saline (PBS) into a measuring cylinder. Pour into the conical flask containing 4g of paraformaldehyde. Cover with parafilm and transfer to t ...

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CELESTINE BLUE SOLUTION

Celestine Blue 2.5g Ferric Ammonium Sulphate (Iron Alum) 25g Glycerin 70ml Distilled water 500ml Dissolve ferric ammonium sulphate in cold distilled water. Add celestine blue and boil for a few mins. ...

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Fixatives

For all these fixatives the most common protocol is to fix then rinse in TBS exchange TBS for 100% methanol and store samples in the freezer where they will be stable for many months (years?). Methano ...

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SECTION ADHESIVES

It is advisable to use an adhesive to promote tissue attachment to the glass slides used in histological preparation. This is routinely achieved by the application of a smear of glycerin/albumen mixtu ...

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Sectioning stained embryos.

The protocols for plastic and wax sections as used by the Vize lab. These protocols work but they have not been optimized. If anyone has better protocols please let us know. Samples. Stain samples str ...

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TISSUE PROCESSING AND PREPARATION FOR SECTIONING

It may be helpful to read the guidelines on the collection of pathological samples. Fixation: Specimens may be routinely fixed in 10% neutral buffered formalin (NBF). Fixation should be commenced as s ...

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Tissue microarray

Creating an interactive database for image viewing and data input.The major problem of using tissue microarray is how to simultaneously examine each core at the microscope know its identity and input ...

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Cryostat Sectioning

Make sure the temperature of the main cryostat chamber is at �150C and that the quick freeze compartment is at least -400C. Always carry your tissue samples across in liquid nitrogen Remove the blade ...

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FREEZING TISSUE FOR CRYOSTAT SECTIONING

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...

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FREEZING TISSUE FOR CRYOSTAT SECTIONING

All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc placing the ...

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Tissue sectioning: Cryostat sectioning method

Overview(ala Fagotto & Gumbiner 1994) MaterialMEMPfa: 0.1M MOPS -- pH 7.4 2mM EGTA 1mM MgSO4 (typically this is made up as 10X MSalts. 4% paraformaldehyde (diluted from a frozen stock of 20%) (st ...

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