显微镜

Hematoxylin StainSolutionsHematoxylinHematoxylin6.4gAmmonium alum60gEthanol200mlGlycerol160mlDistilled water640mlMix for a day then allow the solution to ripen in the dark for at least a month before ...

ACETYL CHOLINESTERASE SECTIONS Unfixed cryostat or cold Formol calcium/gum sucrose fixed sections. SOLUTIONS a. Incubating solution. --acetyl thiocholine iodide 5mg --0.1M acetate buffer (pH.6.0) 6.5m ...

ACID PHOSPHATASE Sections 5-10 micron unfixed or cold formol calcium/gum sucrose fixed Cryostat sections.Stock solutionsA) Pararosanilin-HCL StockPararosanilin 1gDistilled water 20mlHCl Conc 5mlThe pa ...

Stain for Sperm Acrosomes Procedure 1. After sperm preparation adjust sperm concentration as high as possible (5-10 x 106/ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. ...

ADENOSINE TRIPHOSPHATASE (ATPASE) SECTIONSUnfixed cryostat or cold Formol calcuim/gum sucrose fixed on muscle biopsy sections.SOLUTIONSa. 0.1M Glycine buffer.0.75g glycine + 0.585g NaC1 made up to 100 ...

The following is a procedure we have used and modified for detecting DNA synthesis in cells of mouse mammary glands I. BrdU TreatmentMice were injected i.p. with 0.01 mL/g bromodeoxyuridine (Amersham ...

ACID PICRO MALLORY 1. Bring sections to water 2. Stain in Celestine blue 3 mins 3. Wash in water 4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water 6. Blue in Scotts tap water 7. Wash in water 8. ...

The following is a procedure we have used and modified for detecting DNA synthesis in cells of mouse mammary glands I. BrdU TreatmentMice were injected i.p. with 0.01 mL/g bromodeoxyuridine (Amersham ...

Elastic Picro Sirius Red 1. Bring sections to water 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5. Wash in water 6. Wash in 70% alcohol (optional) 7. Stai ...

ELASTIC VAN GIESON (EVG) 1. Bring sections to water. 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5 Wash in water 6. Wash in 70% alcohol (optional) 7. Stai ...

ALKALINE PHOSPHATASE SECTIONSFresh Frozen (jejunal Biopsy) or cold Formal calcium/Gum Sucrose fixed Cryostat 5-7micron thick.SOLUTIONSa. 0.2M Tris HCL buffer pH 8.30.2M Tris (2.42g/100) 25ml 125ml )0. ...

Special Stains in HistologyMucin stainsThere are a variety of mucin stains all attempting to demonstrate one or more types of mucopolysaccharide substances in tissues. The types of mucopolysaccharides ...

ALCIAN BLUE TECHNIQUE SECTIONS4micron Paraffin wax sectionsSOLUTIONSAlcian Blue pH 3.1Alcian Blue 1g0.5% Acetic Acid 100mlAlcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100mlAlcian Blue pH 1.0Alcian B ...

CRESYL FAST VIOLET TECHNIQUE FOR NISSL SECTIONS8µm paraffin wax sectionsSOLUTIONSSTAINING SOLUTIONCresyl Fast Violet-1g Distilled water-100cm3 Acetic acid-0.25cm3METHODTake sections to water Stain in ...

（一） 原理 免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电子显微镜下观察，由于标准物形成一定的电子密度而指示出相应抗原所在的部位。免疫电镜的应用，使得抗原 ...

（一） 原理 免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电子显微镜下观察，由于标准物形成一定的电子密度而指示出相应抗原所在的部位。免疫电镜的应用，使得抗原 ...

Wash glass slides in detergent for 30 minutes.Wash glass slides in running tap water for 30 minutes.Wash glass slides in distilled water 2x5 minutes.Wash glass slides in 95% alcohol 2x5 minutes.Air dr ...

Dissolve 5g of gelatine in 1 litre of distilled water. Do not heat over 60C.When the gelatine has dissolved add 0.5g of chrome alum (chromic potassium sulphate).Cool and dip racks of slides or coversl ...

Fix samplesin MEMFA and then into Dent's Fix (20% DMSO/80% Methanol) If specimens need to be bleached bleach in Curis Bleach for 1/2 hour under white light. Store specimens at -20°C in 100% methanol p ...

Many surgical specimens contain calcified areas which need to be decalcified before processing and sectioning. This is achieved as follows.When the specimen is sufficiently fixed the selected tissue i ...