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        ACID PICRO MALLORY

        互联网

        1192

         

        ACID PICRO MALLORY

         

        1. Bring sections to water

        2. Stain in Celestine blue, 3 mins

        3. Wash in water

        4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water

        6. Blue in Scotts tap water

        7. Wash in water

        8. Rinse in 95% alcohol

        9. Stain in Picro-orange 2 mins

        10. Wash in water until only red blood cells are yellow

        11. Stain in acid fuchsin 5 mins

        12. Wash in water

        13. Stain in equal parts Picro-orange and 80% alcohol

        14. Differentiate in phosphomolybdic acid for 8 mins

        15. Wash in water

        16. Stain in analine blue, 8 mins

        17. Wash in water

        18. Dehydrate, clear . Mount sections in DPX

         


        RESULTS :

        --Fibrin - Bright red

        --Muscle - Red

        --Collagen - Blue

        --Red blood cells - Yellow/Orange

         


        SOLUTIONS :

        1. 0.2% orange G in saturated alcoholic Picric Acid

        2. 1% Acid fuchsin in 3% Trichloroacetic Acid

        3. 1% Phosphomolybdic Acid

        4. 2% Analine blue W5 in 2% Acetic Acid (soluble Blue)

        NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

         

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