实验技能SOP

先制备坐骨神经-腓肠肌标本，而后利用计算机采集系统输出电信号，并将标本与张力传感器相连。利用电信号对标本进行刺激，并通过张力传感器将肌肉的收缩信号输入计算机。 当给予肌肉标本一个高于阈刺激的单刺激时，可以在计算机中观察到由潜伏期、收缩期和舒张期组成的单收缩曲线。当给予两个等强度的阈上刺激，且刺激的间隔大于单收缩的时程，在计算机中可以观察到两个独立的收缩曲线；当给予两个等强度的阈上刺激，且刺 ...

1.麻醉后将头部同定于动物实验用立体定向架卜，切开有额顶头皮，用微型钻磨去颅骨形成5ram直径大小网形骨窗2.在手术显微镜下剪开硬脑膜，用微量注射器将10％BDA溶液(美国Molecular Probes公司产品)缓慢注入右侧的感觉运动区皮质(sensorimotor cortex)内。注射时将微量注射器同定在一 体定向支架上，选择无血管区作为注射点，每个注射点分别在距皮层表面0．5、1．0和2． ...

Instruments For ElectrophysiologyProducts include microelectrode voltage and current clamp amplifiers perfusion chambers perfusion heating systems perfusion steppers glass capillary ...

点击浏览该文件详细说明如下：1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those in turn are plated on glass coverslips pre-coated with poly-D-lysine and laminin ...

Proteasome Preparation from Human BrainYou will need:- Chicken gamma globulin (1mg/ml in H2O). Chicken gamma globulin standards:- Gamma Globulin StandardsFinal Quantity of Gamma GlobulinGamma Globulin ...

These instructions relate in detail how we prepare neuromelanin (NM) from PC12 cells and the conditions used for electron paramagnetic resonance (EPR) detection of NM. Please see the accompanying arti ...

一种极为高效且很方便的转染方法!是华人科学家做出的结果以下是全文 以下内容需要登录后才能完全查看，请您登录马上登陆| 企业会员注册 ...

Productionofneuron-preferentiallentiviralvectors 以下内容需要登录后才能完全查看，请您登录马上 ...

Purpose:The following steps will prepare mounted slices of fixed brain from frozen tissue.Slicing a frozen brain will allow sections as thin as a few μm.These preparations can be used instainin ...

DescriptionFrom the lab of Lucien T. "Tres" Thompson Ph.D. The University of Texas at DallasProtocol for Radial-Arm Maze Training for Unit Recording ...

ACSF FOR USE WITH AGAROSE (Modified from Kleinfeld and Delaney 1996;designed to be heated to 100oC without clouding agarose)MW g/L142 mMNaCl58.5 g/M8.30 g/L5 mM KCl74.6 g/M0.370 g/L10 mM ...

DescriptionFrom the lab of Lucien T. "Tres" Thompson Ph.D. The University of Texas at DallasProtocol for Dissociated Neurons ...

From the lab of Lucien T. "Tres" Thompson Ph.D. The University of Texas at DallasEdited by Barbara Gibbons (2004).&n

DescriptionFrom the lab of Lucien T. "Tres" Thompson Ph.D. The University of Texas at DallasNisslStainingfor Neurons ...

Protocol for Caloric Restriction of Rats v. 1.0very roughly outlined by Tim Goble in another protocolmodified and completed by Tres Thompson Aug. 2005Rationale: Restricting caloric intake of adult rat ...

Version 1.0Edited & expanded by Ron CowartAdapted from an earlier version author unknown ...

Descriptionby Lucien T. "Tres" Thompson Ph.D. The University of Texas at DallasBARNES-STYLE HOLE-MAZE AVOIDANCE LEARNINGSpatial learning tasks for rats and mice are a well recognized as methods of qua ...

Substrate Preparation1. Prepare culture plate by coating with poly-D-lysine (0.15 ml/cm2 50 μg/ml 135 kD) 1-20 hr. and rinse one time with 18Mohm deionized water and let dry.Preparation of Isolated Ne ...

1. From the tube with the brain tissue remove 1 ml medium and save for step 3 being carefulnot to remove the tissue.2. With 1 ml pipettor with sterile blue plastic tip or silanized 9 inch pasteur pipe ...

These instructions relate in detail how we prepare neuromelanin (NM) from PC12 cells and the conditions used for electron paramagnetic resonance (EPR) detection of NM. Please see the accompanying arti ...