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ACSF FOR USE WITH AGAROSE (Modified from Kleinfeld and Delaney 1996;designed to be heated to 100oC without clouding agarose)
| MW | g/L | |
| 142 mM NaCl | 58.5 g/M | 8.30 g/L |
| 5 mM KCl | 74.6 g/M | 0.370 g/L |
| 10 mM Glucose | 180 g/M | 1.80 g/L |
| 10 mM Hepes, Na Salt | 260 g/M | 2.60 g/L |
| Adjust pH to 7.4 | ||
| 3.1 mM Ca ++ Stock | 3.1 ml of 1M | |
| 1.3 mM Mg ++ Stock | 1.3 ml of 1M |
Bring total volume to 1 liter.
ACSF from Llinas Laboratory ( or in vitro perfusions)
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