实验动物模型

AP Assay and FGF Binding Mike NaskiCOS-7 cells were transfected by a standard DEAE-dextran method with 4 mg of plasmid/10 6 cells. Conditioned medium was harvested 3 6 and 9 days after transfection an ...

DNA from Tail Biopsies1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince).(The tubes must have tight-fitting caps so that there are no leaks in steps 3 and 7 below.) 2. Add 0.5 m ...

Genotyping Transgenic Rodents by PCR This is how we test mice and rats for the presence of the transgene by PCR. It is provided for those investigators who are unfamiliar with DNA genotyping and who n ...

MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1 Straight ScissorEtoh Spray bottle1 Bone Scraper1 bottle of PBS2 Forceps (1 medium 1 fine)5 10 cm platesSacrifice AnimalsPlace mice in ...

Always fill-in a form for each mouse you find/submit and make as many notes as possible about his behaviour health status or gross features. Always weigh the mouse. if mouse is found dead : Almost inv ...

Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inh ...

You need to buy glass slides with frosted sandblasted ends (Fisher Scientific Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges ...

Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimen ...

Forms for submitting specimens to Pathology. Columbia University ICG onlyIn order to have full communication between the originator of the mouse and the pathologist you must fill a form with all the ...

Naming mouse strainsThere are internationally accepted rules to name transgenic and knock-out mice. See the following links: Mouse Nomenclature Rules and Guidelines These are suggestions about how to ...

How to keep track of your mice.Breeding mice can be very challenging particularly when you have to breed complex genotypes.You need a way to identify the mice permanently and a way to record birth gen ...

OUTLINEExperimental splenectomy in the mouse is used to study the role of the spleen in platelet destruction..PROTOCOLGeneral anesthesiainject i.p. 250µl/mouse of the ANESTHETIC mixture for verify th ...

名称：大鼠灌注取脑 标准操作规程(SOP)关键词：大鼠 灌注取脑目的： 完整取出大鼠脑部（取材）操作步骤流程：1)麻醉 2)开胸 3)心脏左心室穿针剪开右心耳 4)生理盐水冲水 5)4%多基甲醛固定 6)取脑 7)保存或切片.1 多聚甲醛的配置：一般方法为：4％多聚甲醛PBS缓冲液配法：称取40g PFA溶于装有500ml DEPC水的玻璃容器（烧杯或烧瓶）中，持续加热磁力搅拌至60～65℃，使成 ...

名称：豚鼠饲育管理标准操作规程(SOP)关键词：豚鼠饲育管理目的：本操作规程是关于怎样饲育豚鼠才能使其被最有效的用于实验的具体操作规程。主体内容：1．饲育管理指标(1)环境卫生管理的指标(2)外观健康检查指标(3) 生产数量指标 按平面饲养设备，每人负责饲养管理种母为150只，种公30只，育种60只(其中母种50只，公种10只)：，月上交任务120只(月单位生产指标o．8只)。(4)日粮指标 成令 ...

1、 选取7~8周龄雌性小鼠，阴道口封闭，作为供体，下午3:00左右，每只小鼠腹腔注射PMSG（10 IU）。2、 47~48小时后，每只小鼠腹腔注射HCG（0.8 IU），并与正常公鼠合笼；另取数只适龄母鼠（2月龄以上）作为受体，阴道口潮红，与结扎公鼠合笼。3、第二天上午9:00前观察供体、受体，有精栓者拿出备用。受体笼拿出作好隔离措施。4、10:30左右，断颈处死供体，手术取出整个输卵管，放入 ...

麻醉（anesthesia）的基本任务是消除实验过程中所至的疼痛和不适感觉，保障实验动物的安全，使动物在实验中服从操作，确保实验顺利进行。一、常用的麻醉药（一）常用局部麻醉剂：普鲁卡因，此药毒性小，见效快，常用于局部浸润麻醉，用时配成0.5％～1％；利多卡因，此药见效快，组织穿透性好，常用1％～2％溶液作为大动物神经干阻滞麻醉，也可用0.25％～0.5％溶液作局部浸润麻醉。（二）常用全身麻醉剂：1 ...

名称：转基因小鼠模型的建立(SOP)关键词：转基因小鼠目的：在动物体研究转化基因原理：转基因动物是指染色体基因组中整合有外源基因并能遗传给后代的一类动物。整合到动物染色体基因组的外源基因称为转基因。转基因技术则是指制备转基因动物所需的一套技术，它涉及外源基因的构建、载体和受体的筛选、基因导人技术、供转基因胚胎发育的体外培养系统和宿主动物等许多方面。当制备转基因动物时，外源基因可能只整合到动物的部分 ...

Geraldine Seydoux and Andrew Fire Adapted from Seydoux G. and Fire A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. In C. elegans: Modern Biological Analysi ...

MaterialsCaenorhabditis eleganscultures on agar platesE. colistrain OP50NG agar platesC. elegansRinger's solutionMicroscope (preferrably Nomarski interference or phase)Slides and coverslipsProcedur ...

Grow yeast overnight. Spin down 1.5 ml of culture in microfuge tubes. Decant. Add 250 µl of Qiagen buffer P1 and about 250 µl of glass beads to each tube. Beat or vortex on high for 3-5 minutes. Add 2 ...